BW's Bufotenine Dry Tek

Introduction

This Tek was created by Brennendes Wasser.

Thanks to everyone who has contributed or helped experiment to aid in refining/ improving this process:

This Tek is more or less completed. Lab and test results have been confirmed and are very promising. Feel free to contribute. More photo's to follow soon.

Please take your time with your first extraction, although this is an easy Tek you don't want to rush it and make a mistake.

Forum Link[1]

Safety

Review Acetone and citric acid safety information. Verify solvent MSDS, plastic compatibility, and clean evaporation.

This TEK is food safe if food grade materials are used.

Following this advice does not guarantee safety. It is up to each adult individual to make their own personal decisions.

Consumables

• 300ml water/ 600-800ml of distilled water (tap water works fine too)
• 25g Ca(OH)
• 100g of Syrian Rue seeds (B. Caapi should work too but is yet to be tested).
• 1lt ethyl acetate (sometimes sold as "MEK substitute")
• 10-15g of citric acid
• Sodium Carbonate
• 1lt Jar
• Coffee filter, lab filter or cotton balls (with funnel)

Equipment

• Blender
• Kitchen scale
• Coffee filters, support basket, and funnel Or lab filter and vacuum pump.
• Glass jars with lids
• Fridge
• Milligram scale
• Spray Bottle
• Fry Pan and stove

Detailed Process

Heat Seeds

-Place seeds on minimal heat on fry pan, bring it up to heat until they start popping, lower the heat and turn it off once the seeds have stopped popping.

Powder

-Get 100g seeds and 25g of sodium carbonate grind them with a glass blender into a powder. (Check weight on scales and take note).

Add water

-Add 150ml of water by spraying the seeds/ sodium carbonate mix with a spray bottle. Do this until the paste becomes doughy. Mix well.

Dry Paste

-Dry the paste with a fan and optionally low heat.

Defatting

When dry (check by comparing weight previously noted), defat with 3 x 150 ml boiling Naphtha, then decant.

Pull

Extract and pull with 3 x 150 ml DRY Acetone, filter.

Salting

-Add Fumaric acid or citric acid at least 3 g, slowly, then stir vigorously. Let it rest for 12 h, then decant and watch if the Acetone precipitates more, also check if adding more FASA causes further clouding and let the Alkaloid-Fumarate crystals dry off any remaining Acetone. Note using citric acid may negate the need to wait 12h.

Decant & Reclaim

-Decant Acetone

Dissolve Goo

- Dissolve the Alkaloid-Fumarate crystals in 25 ml hot water. Slowly pour this into a solution of 1,85 g Na2CO3 (0,5:1 Na2CO3:Alkaloid-Fumarates) 25 ml water. This will form impure freebase bufotenine. Decant water.

Base

-Extract the brown precipitating freebase Alkaloid freehbase blob with 100 ml portions of 1:3 Ethyl Acetate:Naphtha. Do this until the freebase blob does not shrink anymore. Black powder will remain. Dont combine the pulls, but put them in the fridge separately. The freebase blob may solidify to a ball upon stirring within this process. Break it apart, if it becomes hard, to speed up extraction. If it remains a goo, still just proceed extracting. Place decanted solvent mix from each pull in the fridge for fridge-frecipitation.

Crash/ Filter TEK Complete

Collect your precipitated freebase Bufotenine (decant solvent). The solvent mix itself will still contain Bufotenine. Evaporate the rest to get all the remaining Bufotenine with a slightly lower purity.

Bufotenine yield: 1-3 % to seed weight This is very pure bufotenine 96%+ purity/

Bufotenin recrystalization for 99.99% purity

The best solvent to form crystaline Bufotenin is Xylene. Actually it would be way too unpolar to ever dissolve Bufotenine. As you can heat Xylene above the melting point of Bufotenin, the latter suddenly becomes soluble while liquifying, while the more polar 2 % dark impurities will remain undissolved. This way you can create 100 % purity Bufotenin from 98 % purity Bufotenin retrieved by the former mixture. See the following pictoral:

1 = Break up crude Bufotenine chunks (if even solid) 2 = Heat in Xylene > 140 °C until only brown powder is leftover (see solubility table how much to use) 3 = transfer surfactant Xylene into new Jar and let it sit until surfactant is clear 4 = dry crystaline Bufotenine - much faster if you wash it with low-boiling Naphtha

Note the fumes are highly dangerous for your health and combustible. You should use the required PPE and have very good ventilation (as in outdoors).

Lab Analysis

Coming soon

Further investigations

-Sodium Carbonate is the only base you should use do not use calcium hydroxide or caustic soda/lye.

-Drying the product in the microwave has been tested to a limited compacity and should work.

-Furmaric or citric acid should work and have been successfully tested.

-Ethyl acetate instead of acetone for the initial pull should work.

Reuse

Reusing solvents is encouraged at the DMT nexus.

Wash spent extract with sodium carbonate saturated water (35% by weight). About 1/5 of the solvent volume as saturated water is enough. Shake vigorously (emulsions do not form). CO2 bubbles may be visible during citric acid neutralization. Keep an eye on any bubbles and release any pressure buildup regularly. Neutralization can be optionally verified with pH paper. Rest washed solution until clear (up to a day or more).

Remove the water layer with a pipette or separatory funnel. Freezing the solvent and filtering the ice slush that forms with a metal strainer is also an option. Finally, decant off any excess solid sodium carbonate/citrate. CaCl2 (sold as deicer, calcium hardener for pools and sometimes is the only ingredient in moisture absorbers tubs) can be used to ensure there is no water still left in the EA.

References

Ethyl Acetate Safety[2] Citric Acid Safety [3] Reusing Solvent [4]