Difference between revisions of "Shaggy's Jungle Tek"

Revision as of 23:27, 18 February 2009

Foreword

Basically we are going to use muratic acid to get the alkaloids from the bark into an aqueous solution. Then we are going to add sodium hydroxide to the solution to: 1) further break down the cell walls of the plant matter releasing more alkaloids into the solution and, 2) convert the alkaloids to their freebase form so they will be soluble in nonpolar solvents. Then we will remove the alkaloids from the solution with a nonpolar solvent (toluene). This orange extract contans DMT (white) and Yuremamine (red). DMT is soluble in heptane while yuremamine is not, so we wash the orange full-range extract with heptane, separating the two compounds. Here are step-by-step instructions.

Extraction guide

Materials

• 500 grams Mimosa Hostilis Root Bark..... Try to find the powdered pink-purple inner root bark if you can. The powder saves you alot of time.
• Toluene (Ace Hardware brand Toluol in the paint thinner section) Xylene may be substituted for Toluene.
• Heptane (Bestine brand, found at Hobby Lobby and Michael's as a rubber cement thinner)
• Sodium Hydroxide (Roebic brand crystal drain opener, found at Lowe's Home Improvement in the plumbing section)
• Muratic Acid (this is concentrated HCl; you'll find this next to the paint thinner at lowe's)
• A 6-liter crock-pot (wal-mart)
• A good blender. (Wal mart) When you choose your blender, look at the mechanism that connects the rotating blade to the motor. You want metal on metal. If this mechanism is plastic, it will break when you grind your bark.
• A pyrex dish that will fit inside your crockpot. (wal-mart)
• A large pyrex dish with the biggest surface area possible. (wal-mart)
• Test tubes, test tube stoppers and test tube clamps, all available at hobby lobby next to the chemistry sets. You want a stopper with a hole in it.
• Pipettes and eye droppers, also at hobby lobby next to the test tubes.
• A glass turkey baster
• A 1-gallon wine jug. Carlo Rossi is cheap and has the perfect shape.
• Full-range pH papers. Get them from a lab supply. The ones at hobby lobby are worthless.
• Mason jars. 1 quart and 1 pint.
• Glass petri dishes
• A large funnel and a large rubbermaid tub to catch spillage.
• A digital scale that goes up to 500 grams and down to .01 g

It would be useful to get a basic lab setup. You can get by with the above materials, but using the proper utensils makes the process more enjoyable and runs smoother. The above is what I use now that I am constantly on the road and my lab must be small and mobile.

Get a basic laboratory glassware set, the laboratory hardware set, and the basic laboratory tool set, and the vacuum filtration kit and a separator funnel.

Get all this stuff, if you are serious about extracting and want to move on to other plants, like san pedro, syrian rue, kratom, etc.

Acid/Base Extraction

• Rip 500g of bark to pieces with some pliers or wire cutters. Then grind the bark a little at a time in the blender. You should be left with stringy fibers and powder. Place this in the crockpot with one gallon of distilled water. Use a glass eyedropper to add muratic acid to the mix and stir it in until you reach pH 2. Use caution when dealing with muratic acid. Avoid skin contact and do not inhale the vapor. Turn the crockpot on high and let it boil for at least 6-8 hours. Stir regularly. Make sure the pH stays at 2, and if it rises, add more acid.
• In a mason jar (or erlenmeyer flask if you have one) place 250ml of warm distilled water. Slowly stir in 200g of sodium hydroxide. This is an exothermic reaction, meaning it releases heat. The water will get very hot very quickly so be careful. Avoid getting sodium hydroxide solution in your eyes or on your skin. Make sure all the powder is dissolved. Pour this solution in the bottom of your gallon wine jug.
• Place the gallon jug in your rubbermaid tub to catch spillage. I usually cut the bottom off of a gallon water jug to use as a large funnel, and then duct tape it upside-down to the mouth of the wine jug. You will still want someone to hold the funnel for you while you pour. Pour the contents of the crockpot into the jug. You want the bark and liquid to all go into the jug. You may have to pick up the bark fibers and stuff them into the jug after you pour off the liquid. Just get it all in there. You want to fill the jug right up to the point where the neck of the jug meets the body of the jug. The neck will be later filled with solvent. If the level does not reach this point, add warm distilled water until it does. Cap the jug and gently invert it upside down and then right side up again several times, so as to mix the bark solution with the sodium hydroxide but not shake it up and create foam. Foam is bad. The pH of this solution should be 14. The contents of the jug should be HOT.

Polar/Nonpolar Extraction

• While the jug is still hot, add toluene. You should have left the neck of the jug empty. Fill the neck with toluene, where the level comes up to about 1/4 inch from the mouth of the jug. Cap the jug, and gently invert the jug for about 10 minutes, mixing in the toluene but not creating foam. Then set the jug on a table and allow the layers to separate completely. This should happen quickly, but it could take hours. Just patiently let it happen. When the layers have separated, use your baster and your pipettes to siphon off the toluene layer, and place it in a mason jar MAKE SURE you don't suck up ANY of the dark bottom layer; this is toxic.

• Repeat this polar/nonpolar extraction at least 3 more times, combining the toluene in a mason jar. If the toluene from the 4th extraction is still dark yellow, do a few more until it is coming up lighter colored.

Evaporation

There are many ways to evaporate toluene. Keep in mind that toluene is very flammable, and inhaling the vapors is also dangerous. Do this in a well ventilated area or outside to avoid explosions or asphyxiation.

• The easiest way is to pour the toluene in a large pyrex baking pan and set it outside with an electric fan blowing on it. The greater the surface area, the better. It evaporates much easier than water. This will not work if it is too cold or humid outside. If this is the case and you must do it inside, pour the toluene into flat bottomed pyrex container with a large surface area that will still fit in your crockpot. Place it in the crockpot with no water, turn the pot on high and place the lid on until it gets nice and hot, then remove the crockpot lid and let a fan blow on the liquid. Replace the lid to let it heat up again, remove the lid again, and so on until the liquid has evaporated. Make sure you do this near a window.

• If you are using heat to evaporate, you will be left with a reddish yellow oil which will solidify as it cools. If it won't solidify, scrape it around with a razor. Move the oil around, scraping it up and spreading it out and scraping it up again, until all the oil had been moved around. Place it under a fan until it hardens.

• Scrape up the solids and place them in a glass petri dish. Place this in the crockpot with no water on high with the lid on. The idea is to melt down the solids and evaporate any remaining toluene because it is toxic and it will prevent the white and red from separating in the next step. Smell the oil every few minutes as it cooks in the crockpot. It should not smell like solvent AT ALL. First it will smell like solvent, then it will smell like mothballs, then it will smell strongly of DMT and nothing else. Blow on the oil while it is in the crockpot so it moves all around and evaps every last bit of solvent.

• When it smells right, set the petri dish in a cool dry place until it hardens. Let a fan blow on it. When it is dry scrape it up with a razor. This is the orange full-range extract. I personally prefer this product, and most of my friends do as well. But if you want pure white DMT, proceed to the next step.

Separation of the alkaloids

For this process, it is important that NO TOLUENE is allowed into the solution. Do not use pipettes or containers that have had toluene in them. It will prevent the white and red from separating.

• Heat some heptane by pouring it in a small mason jar with NO LID. An erlenmeyer flask is ideal. Place the jar inside the crockpot with no water, on high, and put the lid on the crockpot.

• Fill a test tube half way up with your orange extract. If you have more extract remaining, use a second test tube. Use the stopper with the hole in it to avoid pressure buildup. Place the tube in the crockpot, using the test tube clamp to hang off the edge of the crockpot, keeping the test tube upright while inside. Replace the crockpot lid.

• When the heptane is hot and the extract has melted in the bottom of the tube, use a CLEAN pipette to fill the test tube with heptane. Then, using the pipette, suck up some of the heptane from the tube, and then squirt it forcefully down to the bottom of the tube, causing the oil in the bottom to thoroughly mix all throughout the solution. Then place the tube back in the crockpot, upright, and allow the layers to separate. This should happen quickly.

• It is important to keep the solution hot. You should see a bottom red layer and a top yellowish layer. The top layer is supersaturated, so as soon as it begins to cool you will notice the white DMT crystals falling out of the heptane back into the red layer. You don't want this, so keep it hot.

• When the layers have separated, quickly siphon off the heptane layer with a pipette and squirt it onto the large pyrex baking dish, leaving the red oily layer in the tube Refill the test tube with hot heptane, mix the layers again, separate the layers, squirt the heptane in the dish. Do this separation three times total, and squirt the heptane in the dish each time. It will evaporate quickly because it is hot and it is a very small amount of supersaturated solution. Allow this to sit for a few hours. This is your yellow-white DMT. The "red jungle spice" is still in the test tube. Put the tube back in the crockpot so it gets nice and melted, then quickly pour this oil onto a plate to dry. Notice that all the dirt and lint that contaminated the solution is left in the bottom of the test tube.

• Allow the plates with your two products to sit out, exposed to the air, for a few hours to completely dry out and harden. Then scrape them up and put them on petri dishes, put them in the crockpot, and cook out the heptane just as you did the toluene earlier. When the products no longer smell of heptane at all, cool them with a fan, scrape them up, and you are done. These are your finished products. I usually get around 8 grams total product from 500 grams of bark.